Chromatin analyses of Zymoseptoria tritici : Methods for chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq)
نویسندگان
چکیده
منابع مشابه
Chromatin Immunoprecipitation-based Sequencing (ChIP-Seq) on the SOLiD System
SoliD System chip-Seq Analysis The SOLiD System’s ability to generate over 400 million sequence tags (35 bp sequence reads) in a single run enables whole genome ChIP analysis of complex organisms. Sequence tags are mapped to a reference sequence and counted, to identify specific regions of protein binding. The ultra high throughput of the system provides researchers with the sensitivity and the...
متن کاملChromatin immunoprecipitation sequencing (ChIP-Seq) on the SOLiDTM system
and negative control libraries are created, the samples are sequenced on the SOLiD System. The short sequence reads from the SOLiD System are mapped against genomic sequences using the SOLiD System alignment tools available through the Applied Biosystems software development community (http://info.appliedbiosystems. com/solidsoftwarecommunity/) or third-party tools compatible with SOLiD sequenc...
متن کاملFully automated high-throughput chromatin immunoprecipitation for ChIP-seq: Identifying ChIP-quality p300 monoclonal antibodies
Chromatin immunoprecipitation coupled with DNA sequencing (ChIP-seq) is the major contemporary method for mapping in vivo protein-DNA interactions in the genome. It identifies sites of transcription factor, cofactor and RNA polymerase occupancy, as well as the distribution of histone marks. Consortia such as the ENCyclopedia Of DNA Elements (ENCODE) have produced large datasets using manual pro...
متن کاملA high-throughput ChIP-Seq for large-scale chromatin studies
We present a modified approach of chromatin immuno-precipitation followed by sequencing (ChIP-Seq), which relies on the direct ligation of molecular barcodes to chromatin fragments, thereby permitting experimental scale-up. With Bar-ChIP now enabling the concurrent profiling of multiple DNA-protein interactions, we report the simultaneous generation of 90 ChIP-Seq datasets without any robotic i...
متن کاملCharacterization of the Escherichia coli σS core regulon by Chromatin Immunoprecipitation-sequencing (ChIP-seq) analysis
In bacteria, selective promoter recognition by RNA polymerase is achieved by its association with σ factors, accessory subunits able to direct RNA polymerase "core enzyme" (E) to different promoter sequences. Using Chromatin Immunoprecipitation-sequencing (ChIP-seq), we searched for promoters bound by the σ(S)-associated RNA polymerase form (Eσ(S)) during transition from exponential to stationa...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Fungal Genetics and Biology
سال: 2015
ISSN: 1087-1845
DOI: 10.1016/j.fgb.2015.03.006